Volume 12 | Issue 1

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Extraction, Purification and Characterization of Fish Chymotrypsin from Catla catla

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In Aquaculture and Fisheries production of food is important with nutritional security. Developing aquaculture increase the demand of good quality food production. Usually in aquaculture fishmeal is used as feed but due to cost-effectiveness and availability animal protein-based fish feed are not convenient to fish farmers. So, its a need of time that aquaculture industry shift towards plant-based feed formulation with having easily available natural sources. In feed formulation consideration of availability of feed formulation ingredients and cost-effectiveness and rich protein content these are most essential part of formulation. In present feed formulation all these points are considered and feed is formulated. Present feed formulation aquaculture (moringa oleifera) leaves were used as main ingredient along with some other ingredients which are also rich in protein. Moringa leaves are rich in proteins, vitamins and minerals and easily available tropical and subtropical region. The fish feed was accepted by fishes and simply consumed.

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Fish feed, aquaculture, moringa

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The present investigation aimed to develop standard protocol for in vitro propagation of Salvadora persica L. It is one of the important medicinal plants belongs to family salvadoraceae. Nodal segment explants were inoculated using MS medium along with various concentration of growth hormone. The regeneration of multiple shooting was achieved on MS medium along with 0.5 mg/L of IBA in combination of various concentrations of BAP and KIN. The maximum percentage of regeneration and maximum length of shoot were recorded on 0.5 mg/L of IBA in combination of 2.0 mg/L and 2.5 mg/L of BAP and KIN using nodal segment explant respectively.

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In vitro, MS, Salvadora persica L, IBA, BAP, KIN, mg/L.

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The present research paper studies and analyze the agricultural land use at the macro level in Sangamner tehsil. This study is based on secondary data collected from revenue records and district gazetteer offices. Agricultural landuse was depending on physical, climate, socio-cultural, economic, technological and organizational factors. Endeavour is made to study landuse patterns in Sangamner tehsil of Ahmednagar district for the year 1960-61, this is normal year for an agricultural phenomenon. The study region covers 135780 hectares of land and has population of 487939 in 170 villages as per the 2011 Census. Sangamner tehsil is located in the western part of Ahmednagar district. Physiography, rainfall, soil, temperature, and drainage influence on agricultural land use pattern in this tehsil. Rainfall varies between 200 to 225 mms. The present study represents a real situation of cropping pattern in Sangamner tehsil and helps planners and agricultural scientist for agricultural planning at the village level.

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Agriculture landuse, Net Sown Area, Land not Available for Cultivation, Cultivable Waste, Fallow Land, Forest

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SDS-PAGE, Winged bean, protein profiling.

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Biosurfactants are surface active agents produced intracellular / extracellular by microorganisms. The biosurfactants have been reported for similar/better performance in comparison to synthetic surfactants. Their low toxicity, high biodegradability and functional at extreme pH and/or temperature make them green alternative to chemical counterparts. The yeasts, bacteria and some filamentous fungi are capable of producing biosurfactants with different molecular structures. These are generally consisting of fatty acids, glycolipids, lipopeptides, lipopolysaccharides and lipoproteins. Despite of many advantages, biosurfactants have not been fully commercialized due to expensive raw material and low yield. In current studies the efforts are taken to isolate and screen the biosurfactant producing free living diazobacteria followed by optimization of environmental factors for maximum biosurfactant production. The free living diazobacteria isolates were enriched using Burks Nitrogen Free media and screened for biosurfactant production by different techniques viz. hemolysis activity, Drop Collapse test, Oil spreading test and Emulsification Index by E24 % assay. The potent biosurfactant producing diazobacteria isolate was identified with reference to morphology, cultural, biochemical utilization followed by 16S rRNA gene sequencing as Klebsiella singaporensis. On analysing by standard methods, the composition of biosurfactant was found Glycolipid. The higher production of biosurfactant was seen at pH 7.0, 30? C and with 6% inoculum density in 5 days under static conditions. The pre-treated banana peels as a substrate in media had shown higher production of biosurfactant by isolate. The further studies are in progress to identify the biosurfactant using Mass Spectroscopy followed by its different applications for In situ bioremediation.

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Biosurfactant, Bioremediation, Emulsification Index, Surface active agents.

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Spent Mushroom Substrate, Biofertilizers, Enrichment, Radish.

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The phylum Rotifera is the most diverse and dominates the fauna of freshwater bodies. Rotifera is a Phylum of primary freshwater Metazoa. The Nighoj Kund is world-famous for naturally made potholes on the Rock River bed of Kukadi River. This pothole was recorded in the Guinness book of world record. The Kund is located at the Nighoj village, Taluka Parner District Ahmednagar. These potholes are spread on the kukadi river bed up to 3 km long and 10 to 15 Mts., widths and more than 30 Mts., in-depth. The results of the present study show that the Zooplankton Group Rotifers species diversity found from various sites of potholes or Kund water which is belonging to different families such as Asplanchnidae, (01 Specie) Brachionidae, (01 Specie) Trichotriidae (01 Specie) Trichocercidae, (01 Specie) Lecanidae (01 Specie) and Testudinellidae (01 Specie) all the species are belonging to order Ploima and Flosculariaceae. The present study is attempted to find out the diversity of rotifers of Nighoj Potholes (Kund) (M.S.) Parner Taluka Ahamadnagar District (M.S.) India

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Kund, Potholes, Biodiversity, Parner, Rotifers, Zooplankton.

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Presentcommunication deals with some ethno veterinary medicinal uses of 24 plant species. The aim of the study was primarily to evaluate ethno veterinary uses of the plants known to tribals of Toranmal region of Satpuda Mountain. The study area is rich in biodiversity and the tribals of the area possess valuable ethno veterinary knowledge. Conservation of these medicinal plants is needed. Ethno- vetenerical data gathered during botanical exploration of Satpuda region is presented here.

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ethno veterinary, tribal, Toranmal, Satpuda.

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During the present study 16 algal species of algae viz. Microcystis pulveria, Chroococcusmontanus, Gloeocapsapolydermatica, Aphanocapsakoodersi, Aphanocapsabiformis, Synechocystispevalekii, Merismopedia punctata, Chroococcidiopsis indica, Oscillatoria chlorina, Oscillatoria terebriformis, Oscillatoria subbrevis, Oscillatoria vizagapatensis, Phormidium fragile, Phormidium tenue, Lyngbyarubida and Microcoleuschthonoplastes were recoded from the water bodies in Bhokardhan region of Jalna district in Maharashtra. A brief report is presented in this communication.

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Bhokardhan, Waterbodies, Algae, Chlorophyaceae.

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The purpose of this research paper is to explore Six Sigma in Service Sector Specially in Banking Process. Six Sigma is avery powerful toolas to reduce waste and enhance the service process.Though its benefits are limited in service sector rather than manufacturing process. Six sigma deployed in Service sector based on valued and non valued added process. Six sigma can be used in service sector such as banking and health care. This research paper focuses on key performance indicator of six sigma in various stages to cover wide range of Service process specially in Vidharbha region.. Design/Methodology/Approach: The tactic is to create the practical experience and research conducted by the author on six sigma in banking process. Further analysis shows the changeling in implementing Six Sigma in banking by using primary as well as secondary data. Finding: Here a ne w approach has been developed to reach the six sigma level so that customer will be satisfied. DMAICM is the new approach of Six sigma methods the leading new phase and its affection and reality will be described by using primary as well as secondary data. Practical Implication: This research paper is used for academic who are really interested in six sigma process. This paper provide a new concept past, present and future of Six sigma in Service sector. Further this paper express the new approach on systemic ways for implementing the process in service sector.

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Six Sigma, Implementation of Six sigma in Banking, DMAICM process, Banking Process and Continuous improvement.

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Present study is on our own critical observations on fresh plant material collected from the different parts. The observations are also based on herbarium specimens. Relevant data from literature have been referred for comparative study. The study of Carex Linn. provides a detailed taxonomic description, illustration and relevant information for its easy identification in the field. Two species of Carex is elaborately described in this paper. The present report is hoped to provide basic material for further research in Cyperaceae.

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Carex, Floristic, Cyperaceae.

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Green chemistry generally refers to the replacement of hazardous chemicals with more environmentally friendly materials in product manufacturing, use and disposal. By introducing more environmentally benign chemicals into the design and manufacturing processes, green chemistry strives to reduce pollution and the environmental impacts associated with industrial wastes and product disposal. SRC (Selective Catalytic Reduction) is a proven leader in this field, especially in the area of identifying and assessing safer substitutes.Green chemistry is the design of chemical products and processes that reduce or eliminate the use or generation of hazardous substances. Green chemistry applies across the life cycle of a chemical product, including its design, manufacture, use, and ultimate disposal. Green chemistry is also known as sustainable chemistry. Due to increasing human activities pressure on natural resources is increasing day by day and something has to be done to reduce it, because if it continues at this rate life on earth would become unsustainable. Many environmentalist and scientist are working to make certain strategies to reduce environmental pollution. In addition to solid waste which we see in our household garbage bins, there are medical, industrial, agricultural and mining wastes. Environment is polluted mostly by improper disposal of waste. Therefore there is a need to keep a check over waste disposal.

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SRC-Selective Catalytic Reduction, hazardous chemicals, environmental pollution.

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Plants extracts appear to be one of the most effective alternative methods of plant diseases control which are less harmful to human beings and environment. In vitro antifungal activity of methanolic and ethanolic leaf extract of T.cordifolia against three postharvestfruit rotcausing fungal pathogens viz.Aspergillus niger, Rhizopus stolonifer and Penicillium digitatum were tested by using radial growth technique. The result exhibited that both plant extracts had antifungal activity against the tested fungi. It is also proved that by using higher concentration of plant extract, inhibition level of mycelial growth get increased. Methanolic leaf extract of T. cordifolia shows better inhibition of A.niger than Rhizopus stolonifera & Penicilliumdigitatum.

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Medicinal Potential of Tinospora Cordifolia Against fruit rot pathogens.

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Thunbergia erecta, Nectar, sugars, Amino acid, visitors

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The paper presents an analytical study of air quality of Jalna city in which an attempt has been made to prepare an inventory of pollutants (PM10, PM2.5, SO2, NOx) at regional level to know the current cumulative air pollution load in the study area by using Air Quality index determination. Monitoring stations were set up at two different locations i.e. industrial and residential and samples have been analyzed, subsequently air quality index has been computed. The results reveal that gaseous pollutants such as SO2 and NOx are within the permissible limits prescribed by National Ambient Air Quality standards (NAAQS) at both the monitoring locations. It has been observed that the calculated AQIs value for SO2 and NOx falls under good category. PM2.5 levels exceeded the prescribed (NAAQS) and PM10 levels also exceeded the prescribed NAAQS at both the monitoring locations thus particulate matter is the predominant cause of air pollution in the study area. The calculated AQI values of PM10 fall under moderate and poor categories. The calculated AQIs values of PM2.5 fall under moderate and also in poor categories. The overall AQI was found to fall under poor category owing to PM2.5. Thus it is observed that PM2.5 and to some extent PM10 are responsible pollutants at both these locations of Jalna city. Members of sensitive groups like older adults and children may experience health effects like heart or lung disease on prolong exposure and at greater risk compared to general public is affected to lesser extent

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Ambient air quality, Air Quality Index (AQI), Gaseous pollutants- SO2, NOx, Particulate pollutants, RSPM - Respirable Suspended Particulate Matter (PM2.5), NRSPM - Non Respirable Suspended Particulate Matter (PM10), Residential area, Industrial area.

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These fishes are self-perpetuating after its establishment and continuously to reduce mosquito larvae for long time. The cost of introducing larvivorous fish is relatively lower than that of chemical control. Use of fish is an environment friendly method of control. Larvivorous fish such as Gambusia and Poecilia prefer shallow water where mosquito larvae also breed. Guppy is the most popular fish on the commercial basis forming nationwide. The guppy is an excellent choice for beginners and experts alike. This beautiful fish is a perfect choice for first time fish keepers and children. They are very hardy and can survive in conditions where other fish would perish. The guppy is also an excellent choice for cycling new aquariums. Cycling is the term used to describe the period during which the aquarium matures. An aquarium is mature when it contains enough essential bacteria to break down fish waste to a less toxic state. Although Guppy Fishes are aquatic, fertilization is internal and reproduction is viviparous which relate more closely to terrestrial vertebrate reproduction hence we choose this fish for the investigation.

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Guppy Fishes, biological control, length-weight relationship.

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Diversity, Grasshopper, identification and Morphology

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Survey of pteridophytic fern was carried out from Hiranyakeshi hills.The auther are engaged in the study of fern of the Sahyadri hills in weastern ghats. It is a rich biodiversity area in Sahyadri ranges. The present paper deals with the extended and distributed of 05 fern species. Belonging to 04 genera of fern which includes Adiantum, Cheliantus, Athyrium and Pityrogramma and their species. It was collected from origin of Hiranykeshi river hills, Amboli.

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biodiversity, fern, Sahyadri hills, Western ghats,

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In this research paper, enumerate and study the Chlorophyceae (green algae) of water of Darna River. During study period, month wise qualitative and quantitative analysis were carried out and data obtained. Chlorophyceae recorded 27 algal speciesduring study period. In this paper also discuss month wise occurrence of total algal species of class Chlorophyceae at six sampling sites at Darna River during January 2015 to December 2016

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Chlorophyceae (green algae), Darna River

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